AustralieFrV1, Main, Exploration, bibRecord, 000D96

Doping control study of AICAR in post-race urine and plasma samples from horses.

Identifieur interne : 000D96 ( Main/Exploration ); précédent : 000D95; suivant : 000D97

Doping control study of AICAR in post-race urine and plasma samples from horses.

Auteurs : Jenny K Y. Wong [République populaire de Chine] ; Wai Him Kwok [République populaire de Chine] ; George H M. Chan [République populaire de Chine] ; Timmy L S. Choi [République populaire de Chine] ; Emmie N M. Ho [République populaire de Chine] ; Murielle Jaubert [France] ; Ludovic Bailly-Chouriberry [France] ; Yves Bonnaire [France] ; Adam Cawley [Australie] ; H. Ming Williams [Australie] ; John Keledjian [Australie] ; Lydia Brooks [Canada] ; Adam Chambers [Canada] ; Yuanyuan Lin [République populaire de Chine] ; Terence S M. Wan [République populaire de Chine]

Source :

Drug testing and analysis [ 1942-7611 ] ; 2017.

RBID : pubmed:28407446

Abstract

Acadesine, 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside, commonly known as AICAR, is a naturally occurring adenosine monophosphate-activated protein kinase (AMPK) activator in many mammals, including humans and horses. AICAR has attracted considerable attention recently in the field of doping control because of a study showing the enhancement of endurance performance in unexercised or untrained mice, resulting in the term 'exercise pill'. Its use has been classified as gene doping by the World Anti-Doping Agency (WADA), and since it is endogenous, it may only be possible to control deliberate administration of AICAR to racehorses after establishment of an appropriate threshold. Herein we report our studies of AICAR in post-race equine urine and plasma samples including statistical studies of AICAR concentrations determined from 1,470 urine samples collected from thoroughbreds and standardbreds and analyzed in Australia, France, and Hong Kong. Quantification methods in equine urine and plasma using liquid chromatography-mass spectrometry were developed by the laboratories in each country. An exchange of spiked urine and plasma samples between the three countries was conducted, confirming no significant differences in the methods. However, the concentration of AICAR in plasma was found to increase upon haemolysis of whole blood samples, impeding the establishment of a suitable threshold in equine plasma. A possible urine screening cut-off at 600 ng/mL for the control of AICAR in racehorses could be considered for adoption. Application of the proposed screening cut-off to urine samples collected after intravenous administration of a small dose (2 g) of AICAR to a mare yielded a short detection time of approximately 4.5 h. Copyright © 2017 John Wiley & Sons, Ltd.

DOI: 10.1002/dta.2205
PubMed: 28407446

Affiliations:

Le document en format XML

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<author><name sortKey="Ho, Emmie N M" sort="Ho, Emmie N M" uniqKey="Ho E" first="Emmie N M" last="Ho">Emmie N M. Ho</name>
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<author><name sortKey="Jaubert, Murielle" sort="Jaubert, Murielle" uniqKey="Jaubert M" first="Murielle" last="Jaubert">Murielle Jaubert</name>
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<author><name sortKey="Bailly Chouriberry, Ludovic" sort="Bailly Chouriberry, Ludovic" uniqKey="Bailly Chouriberry L" first="Ludovic" last="Bailly-Chouriberry">Ludovic Bailly-Chouriberry</name>
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<author><name sortKey="Bonnaire, Yves" sort="Bonnaire, Yves" uniqKey="Bonnaire Y" first="Yves" last="Bonnaire">Yves Bonnaire</name>
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<author><name sortKey="Brooks, Lydia" sort="Brooks, Lydia" uniqKey="Brooks L" first="Lydia" last="Brooks">Lydia Brooks</name>
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<author><name sortKey="Chambers, Adam" sort="Chambers, Adam" uniqKey="Chambers A" first="Adam" last="Chambers">Adam Chambers</name>
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<wicri:regionArea>Equine Drug Evaluation Centre, Canadian Pari-Mutuel Agency, 115 Sunnyridge, RR#1, Jerseyville, Ontario, L0R 1R0</wicri:regionArea>
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<front><div type="abstract" xml:lang="en">Acadesine, 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside, commonly known as AICAR, is a naturally occurring adenosine monophosphate-activated protein kinase (AMPK) activator in many mammals, including humans and horses. AICAR has attracted considerable attention recently in the field of doping control because of a study showing the enhancement of endurance performance in unexercised or untrained mice, resulting in the term 'exercise pill'. Its use has been classified as gene doping by the World Anti-Doping Agency (WADA), and since it is endogenous, it may only be possible to control deliberate administration of AICAR to racehorses after establishment of an appropriate threshold. Herein we report our studies of AICAR in post-race equine urine and plasma samples including statistical studies of AICAR concentrations determined from 1,470 urine samples collected from thoroughbreds and standardbreds and analyzed in Australia, France, and Hong Kong. Quantification methods in equine urine and plasma using liquid chromatography-mass spectrometry were developed by the laboratories in each country. An exchange of spiked urine and plasma samples between the three countries was conducted, confirming no significant differences in the methods. However, the concentration of AICAR in plasma was found to increase upon haemolysis of whole blood samples, impeding the establishment of a suitable threshold in equine plasma. A possible urine screening cut-off at 600 ng/mL for the control of AICAR in racehorses could be considered for adoption. Application of the proposed screening cut-off to urine samples collected after intravenous administration of a small dose (2 g) of AICAR to a mare yielded a short detection time of approximately 4.5 h. Copyright © 2017 John Wiley & Sons, Ltd.</div>
</front>
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<li>Canada</li>
<li>France</li>
<li>République populaire de Chine</li>
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<settlement><li>Sha Tin</li>
</settlement>
<orgName><li>Université chinoise de Hong Kong</li>
</orgName>
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<tree><country name="République populaire de Chine"><noRegion><name sortKey="Wong, Jenny K Y" sort="Wong, Jenny K Y" uniqKey="Wong J" first="Jenny K Y" last="Wong">Jenny K Y. Wong</name>
</noRegion>
<name sortKey="Chan, George H M" sort="Chan, George H M" uniqKey="Chan G" first="George H M" last="Chan">George H M. Chan</name>
<name sortKey="Choi, Timmy L S" sort="Choi, Timmy L S" uniqKey="Choi T" first="Timmy L S" last="Choi">Timmy L S. Choi</name>
<name sortKey="Ho, Emmie N M" sort="Ho, Emmie N M" uniqKey="Ho E" first="Emmie N M" last="Ho">Emmie N M. Ho</name>
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<name sortKey="Wan, Terence S M" sort="Wan, Terence S M" uniqKey="Wan T" first="Terence S M" last="Wan">Terence S M. Wan</name>
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<name sortKey="Bonnaire, Yves" sort="Bonnaire, Yves" uniqKey="Bonnaire Y" first="Yves" last="Bonnaire">Yves Bonnaire</name>
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<name sortKey="Chambers, Adam" sort="Chambers, Adam" uniqKey="Chambers A" first="Adam" last="Chambers">Adam Chambers</name>
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HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D96 | SxmlIndent | more

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{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:28407446
   |texte=   Doping control study of AICAR in post-race urine and plasma samples from horses.
}}

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       | NlmPubMed2Wicri -a AustralieFrV1

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Serveur d'exploration sur les relations entre la France et l'Australie

Doping control study of AICAR in post-race urine and plasma samples from horses.

Doping control study of AICAR in post-race urine and plasma samples from horses.

Source :

Abstract

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	Serveur d'exploration sur les relations entre la France et l'Australie
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